Research Article
U. Harini and A. K. M. Pawar
Abstract
To develop and validate a simple, rapid, accurate and economical UV and visible Spectrophotometric method for determination of capecitabine in bulk and its formulation product. UV and Visible Spectrophotometric method was performed by using UV/Vis double beam spectrophotometer with spectral band width of 1 nm and 1.0 cm matched quartz cells and glass cells were used for UV and Visible regions respectively. Methanol was used as solvent and the maximum absorbance of Capecitabine was observed in methanol at 308nm in UV region and 616nm in visible region respectively. The linear calibration range was found to be 5μg/mL to 30μg/mL in UV and 10μg to 100μg/mL in visible region respectively. The correlation coefficient (R2) is 0.9985 and the regression equation is y=0.0329x- 0.747 in UV region and correlation coefficient (R2) is 0.9989 and the regression equation is y=0.0081x+0.0356 in visible region respectively. The method was validated in terms of Linearity, Precision, Accuracy, Robustness, LOD and LOQ as per ICH guidelines.