Research Article
Ravi Challa and N. V. S. Naidu
Abstract
A simple stability indicating high performance liquid chromatographic method has been developed for the simultaneous determination of amlodipine besylate in combination with metoprolol succinate using reverse phase Hypersil C18 column (150x4.6, 5μ) with UV detection at 230 nm. The mobile phase consisting of potassium dihydrogen phosphate buffer and acetonitrile in a ratio of 65:35 (v/v) PH was adjusted to 6.8±0.1 and at a flow rate of 1.0 mLmin-1.The method was linear over the concentration range for amlodipine 2.5-15 μgml-1 and for metoprolol 12-75 μgml-1. The recoveries of active pharmaceutical ingredient (API) amlodipine besylate and metoprolol succinate were found to be in the range of 100.10 – 101.16% and 98.50-100.02% respectively. The method was validated and was successfully employed for the routine quantitative analysis of pharmaceutical formulations containing amlodipine besylate and metoprolol succinate in combined tablet dosage form.