Research Article
S. Venkat Rao*, M. Srinivasara
Abstract
Literature survey has been revealed that there is no any visible spectrophotometric method has been reported for estimation Bortezomib in bulk form and in pharmaceutical formulation. Hence an attempt has been made to develop and validate a simple, economic, rapid, and accurate method. In present investigation three simple and sensitive extractive UV-Visible spectrophotometric methods have been developed and validated for the determination of bortezomib in formulation dosage form. The developed methods involve formation of non extractable Condensation complex of drug with Anthranilic acid (M-A), 2-chlorophenyl hydrazine (M-C) and Oxidative coupling complex with MBTH/Fe (III) (M-B) dyes in basic and acidic mediums. Non extractable complexes shows maximum absorption for M-A, M-B, and M-C at 485 nm, 630 nm and 560 nm respectively, Beer’s law is obeyed within the limits 2.5-12.5 μg/mL, 5-25 μg /mL and 2.5-12.5 μg /mL and molar absorptivity is found to be 4.630x104 , 1.544x105 and 7.76x104 for method-A, Method-B and Method-C respectively The colored chormophores were found to be stable for 40 min. 60 min and 30 min respectively.