Original Articles
Dennis D. Raga, Annabelle A. H
Abstract
A mixture of bauerenol (1a), a-amyrin (1b) and b-amyrin (1c) in a 3:1:2 ratioobtained from the dichloromethane extract of air-dried leaves of Ardisia squamulosa was tested on duck chorioallantoic membrane (CAM). The mixture of 1a-1c exhibited inhibition of vascularization and formation of ghost vessels on CAMswith 100% embryo survivability at the end of a 9-day treatment period. A possible angio-suppression by inhibition of branch point formation, reduction of inter-capillary distance as well as reduced vascular density in CAMs administered with 1a- 1care also reported. Immunohistochemistry reveals high degree of von Willebrand factor expression and the absence of epithelial membrane antigen. This suggests that the observed angio-suppression was due to the effect of von Willebrand factor in regulation of other angiogenesis modulator