Original Articles
Cai-xia Li, Ying-zi Wang*, Chu
Abstract
To study transdermal absorption mechanism of matrinenanoemulsions gel on skin in mice. Coumarin 6 was used by embedding method as a fluorescent marker of total matrinenanoemulsion and nanoemulsion gel. Distribution of coumarin 6 on skin was analyzed by confocal laser scanning microscopy. The strongest fluorescence was found in epidermis of mice skin. And stronger fluorescence was found in the upper dermis. Fluorescence decreased significantly in deeperdermis. However,fluorescence in the hair follicle and its appendages was stronger than other areas. The fluorescence of total matrinenanoemulsion group in skin layers was stronger than total matrinenanoemulsion gel group and coumarin 6 solution control group. Nanoemulsion could increase permeation of coumarin 6 in each layer of skin. Fluorescence mainly distributed in epidermis, hair follicle and its appendages after administration.