Synthesis of N-(2-nitrophenyl) pyrazine-2-carboxamide and N-(4-nitrophenyl) pyrazine-2-carboxamide and evaluation of their antimycobacterial properties using in vitro and in-silico techniques

Patil S. U. and Ganatra S. H.

Abstract

N-(2-nitrophenyl) pyrazine-2-carboxamide and N-(4-nitrophenyl) pyrazine-2-carboxamide synthesized and studied as an anti-tuberculosis agents using in-silico and in-vitro analysis. In-silico study reveals the inhibition of Mycobacterium tuberculosis mycolic acid cyclopropane synthase CmaA2 (PDB Code : 3HEM) by both compounds. Whereas in-vitro study, reveals the inhibition of M. tuberculosis H37Rv occurred at concentrations ≥32 μg/ml for n- (2-nitrophenyl) pyrazine-2-carboxamide only.