Synthesis of 4-hydroxybenzoic acid incorporated azo dyes derivatives as potent biological activity molecules

Ramesh F. Pagariya and Nandkis

Abstract

Synthesis of most azo compounds involves diazotization of a primary aromatic amine, followed by coupling with one or more nucleophiles. Thus, benzoic, phenolic, salicylic and naphtholic compounds acts as nucleophiles and undergoes coupling reactions. In this study, a series of azo compounds were synthesized in excellent yields via the diazotization of different aromatic amines followed by coupling with 4-Hydroxybenzoic acid, which is a phenol as well as carboxylic acid. These compounds were characterized by elemental analysis, IR, 1HNMR and MASS spectroscopic techniques. The synthesized compounds have been tested in vitro against human pathogens in order to assess their antibacterial potential using disk diffusion method. The compounds analysed for its antibacterial action were found to be potent antibacterial agents at all concentrations against all the tested pathogenic cultures