Original Articles
Zhigang Liu, Peijie Chen, Yuzh
Abstract
Purpose: The aim of the present study was to investigate the impact of semen astragali complanati (SAC) on GAD67, GABA-T and SSADH gene expression in the hippocampus of exercised rats. Methods: All rats were randomly divided into four average groups: a control group, animals receiving no SAC and no training (NSNT), animals receiving moderate training and no SAC (MTNS), animals receiving intensive training and no SAC (ITNS) and animals receiving intensive training and SAC (ITS). The Trizol homogenate of hippocampus was prepared after 7 weeks training for rats. The products of RT-PCR were separated by 1% agarose gel electrophoresis and quantitatively analysised by UVI gel analysis system. The data of each group was processed and tested by S-N-K of one way ANOVA. Results: The GAD67 significantly enhanced in the ITNS group than in the other three groups (p＜0.01, p＜0.01, p＜0.01), and significantly enhanced in the MTNS and ITS group than in NSNT group (p＜0.01, p＜0.01). The GABA-T significantly decreased in the ITNS group than in the other three groups (p＜0.01, p＜0.01, p＜0.01). The SSADH significantly decreased in the ITNS group than in the other three groups (p＜0.01, p＜0.01, p＜0.01), and significantly decreased in the ITS group than in MTNS group (p＜0.01). Conclusions: (1) Intensive exercise significantly up-regulated the expression of GAD67 while down-regulated the expression of GABA-T and SSADH in the hippocampus of exercised rats, which promoted the central inhibition and correlated with exercise intensity. Therefore, this study supports the theory of “protective inhibition” in exercise induced fatigue. (2) SAC can significantly down-regulate GAD67 expression while up-regulate the expression of GABA-T and SSADH and then reduce Glu converting into GABA, improve the whole excitability of rat hippocampus and delay the central inhibition and central fatigue induced by high intensity exercise. (3) The regulating effects of SAC on GAD67, GABA-T and SSADH in the hippocampus of exercised rats may be related to its effect of tonifying the liver and kidney.