Study on saccharification process and strains filtration during the ethanol fermentation of office waste paper

Lin Ma

Abstract

Office waste paper is a kind of renewable resource wich is high in cellulose and cheaper. In this paper, we selected the optimal conditions of the office waste paper atmospheric pressure two-stage acid hydrolysis by single-factor test, and choosing the optimal strains in ethanol fermentation between super saccharomyces cerevisiae、Zy-momonas mobile and candida shehatae. The result showed that the optimum conditions of hydrolysis technology for twostage acid hydrolysis under normal pressure were as follows: in the stage of concentrated acid hydrolysis, acid concentration was 60%, hydrolysis temperature was 65°C, hydrolysis time was 120min and ratio of acid to solid was 18:1; in the stage of dilute acid hydrolysis, hydrolysis temperature was 100°C, hydrolysis time was 180min and ratio of acid to solid was 150:1. Super saccharomyces cerevisiae would be selected for the stage of ethanol fermentation. The optimized conditions were that inoculation amount was 400ml, the culture temperature was 34°C and the culture time was 60h.