Original Articles
Ankita Jain and Hotam Singh Ch
Abstract
The main focus of this study was to isolate some antibiotic producing actinomycetes strains from soil. Soilsamples were taken from various places of the effluent drainage of Malanpur industrial area, Bhind M.P. Eleven soil samples were collected, serially diluted and spread on actinomycetes isolation agar supplemented with amoxicillin and cycloheximide for inhibition of bacteria and fungi, respectively and incubated at 28 ± 2°C for 7-14 days. Twenty-five potential colonies were further purified and stored in agar slants and glycerol stocks. Morphological and biochemical characterization was done and purified isolates were test against pathogenic microorganisms for screening. Selected isolates with antagonistic properties were inoculated in production media for the extraction of secondary metabolites or antimicrobial products. Agar well diffusion was used for antimicrobial activity of crude extracts against Escherichia coli (E. coli), Methicillin-Resistant Staphylococcus aureus (S. aureus) and Vancomycin-Resistant Enterococci (VRE). Extract of selected 25 isolates were shown to be active against most of the selective microorganism. Zone of inhibition were recorded in the range of 15-19 mm. Isolates AGU 25 has been found most effective against all selective microorganisms. It shows strong inhibition activity against Vancomycin-Resistant Enterococci (VRE). All the isolates of this study were gram positive and their colony morphology was studied microscopically. Potent antibiotics from these actinomycetes could contribute a lot to fight against antibiotic resistant pathogens. Selected isolates have been shown strong antimicrobial activity against resistant pathogens.