Research Article
Dhananjay B. Meshram, Priyanka
Abstract
This paper presents an HPTLC method, validated for simultaneous determination of fluconazole (FLZ) and tinidazole (TNZ) in a combined dose tablet. Chromatography was performed on silica gel 60F254 plates with 250 μm thickness, using Toluene: Ethyl acetate: Chloroform: Methanol (1.2:3:2:0.8 v/v) as a mobile phase. For the proposed method, linearity (FLZ-r2 > 0.996; TNZ-r2 > 0.997), sensitivity (FLZ-3.04 ng/spot; TNZ-11.12 ng/spot), recovery (FLZ- 98.66%-99.13%; TNZ- 99.26%-100.54%), and repeatability were found to be satisfactory. The HPTLC method offers a simple, sensitive, rapid and cost effective technique that can be applied successfully in routine quality control of formulations containing FLZ and TNZ.