Original Articles
Güzide Pekcan
Abstract
Allura red, brilliant blue and β-carotene are color substances used in foods. In this study, allura red, brilliant blue and β-carotene were simultaneously determined without any chemical pre-separation by the spectrophotometric method. Chemometric methods have evaluated the obtained experimental data. In the first step, the synthetic mixtures containing a three-color material were examined, and the obtained results were applied to the principle component regression-PCR and partial least squares -PLS. In the next step, using PLS and PCR methods, the quantities of allura red, β-carotene and brilliant blue in commercial beverage samples were measured at the same time. A good fit was found between PLS and PCR methods calculated from experimental data and calibration methods when the results were compared statistically. As a result of the determination, high recoveries and low standard deviations were found. For absorbance and concentration values were used in Minitab and other chemometric programs to calculate estimated concentrations with PCR and PLS.