Separation of nucleotides and inorganic phosphate for quantification of DNA reference materials based on phosphorus analysis using high performance liquid chromatography�??inductively coupled plasma-mass spectrom

Yang Xue

Abstract

Deoxyribonucleic acid (DNA) is a carrier of genetic information but also an organic compound with complex molecular structure [1]. Accurate quantification of DNA is of great importance for many critical areas, including many biological, clinical and microbiological analyses. The most routinely used traditional methods for DNA quantification are UV spectrophotometry (UV) and fluorescent dye, but their sensitivity and stability is still quite limited. Quantitative PCR (qPCR) dramatically improved the limit of detection (LOD), yet is still facing higher risk of false positives. Most importantly, the traceability of DNA concentration remains a challenge in most of the gene analysis labs