Original Articles
Badiadka Narayana, Kumble Divy
Abstract
Simple and sensitive spectrophotometric methods for the determination of midazolam (MID) are proposed. The methods are based on the bromination of the drug using N-bromosuccinimide (NBS) and the excess oxidant is determined by either malachite green (MG) or safranin O (SO), the absorbances of which are measured at 617 or 522 nm for MID-MG and MID-SO, respectively. Under the optimized experimental conditions, Beer’s law is obeyed in the concentration ranges of 15.00-30.00 and 10.00-50.00 μgmL-1 with molar absorptivity of 8.88 × 103 and 1.18 × 104 L mol-1 cm–1 for methods A and B, respectively. The limits of detection for methods A and B are 1.7368 and 0.5823 g mL-1, respectively. No interference was observed from excipients commonly present in formulations. The proposed methods are applied successfully for the pharmaceutical formulations.