RNA Preservation and Stabilization

Zahra Salehi and Mohammad Naja

Abstract

RNA quality and integrity is a prominent issue in gene expression analysis which emerged as a critical tool in life science researches, drug discovery and optimization of bioproduction. Handling and preservation methods including formalin fixation, flash freezing and chemical preservatives (sulfate solution and TRIzol) and commercial compounds (RNAlater, Allprotect and PAXgene) are widely applied to keep high quality RNA within fresh tissue samples. In this article, we tried to give a general idea on basic aspects of the above methods.