Original Articles
Uttam Kumar Neerudu, Swarna Ba
Abstract
In the present study, a higher purity of 1500 IU/mg was obtained with the crude hCG extract which was subjected to a second round of solvent fractionation to extract 12.32 mg of protein with purification. Virus deactivation step was performed here. To purify, column chromatography was used which gave 8.12 mg of protein content with 1500 IU/mg of biological activity.