Original Articles
L. Jeyanthi Rebecca*, S. Sharm
Abstract
Industrial enzymes are produced by various organisms using a wide variety of substrates. The present study was carried out to investigate the production of protease by Aspergillus niger using fish scale as the growth media. Fish scale, the chief waste material of fish processing industries, was enzymatically hydrolysed by protease produced by A. niger. Two mutated strains of A. niger, namely, AB30 and AB60 were also used apart from the wild type A. niger strain. Trichloroacetic acid buffer was used to maintain the optimum moisture content of the substrate. No separate nutrient sources apart from the fish scales were used. This proves the cost effectiveness and efficiency of this process. The enzyme activity was analyzed from the 5th day and it was further analyzed for three consecutive days and the results were plotted and analyzed.