Preparation and toxicity of protein-calcium complex with phosphorylated human-like collagen

Jianjun Deng, Yanru Chen, Chen

Abstract

In order to bind more amount of calcium, human-like collagen (HLC) was first phosphorylated by reacting with D-Glucose 6-phosphate disodium salt hydrate, and then the phosphorylated HLC (PHLC) was chelated with CaCl2 to prepare the PHLC-Ca composite. The Optimum reaction condition for the PHLC-Ca was: pH 7.5, nCaCl2 : nHLC = 600, stirring for 1 h. The Cell Counting Kit-8 (CCK-8) assay indicates that HLC, PHLC and PHLC-Ca were safe to BHK21 and could stimulate the normal growth of BHK21.