Preparation and Photodynamic Properties of Methylene Blue-Silica Nanospheres

Heyong Huang, Yan Ding, Jiahon

Abstract

This article presents the development and characterization of SiO2 nannoparticles loaded with methylene blue (SiO2-MB), which are designed to administered for photodynamic therapy (PDT). Induced by light irradiation, the entrapped MB generated 1O2, and the produced 1O2 was measured with anthracene-9,10-dipropionic acid. In comparison to the unmodified MB, the MB nanoparticles display lower singlet oxygen production but higher DNA photocleavage capability. Furthermore, in vitro experiments illustrated that SiO2-MB was efficiently taken up by tumor cells, and that light irradiation of such impregnated cells resulted in significant cell death. Thus, these properties of SiO2-MB could possibly make it especially promising foruse in clinical photodynamic therapy.