Original Articles
Elakkiya Selvi J and Helen Cha
Abstract
Nano Tecnology employs knowledge from the fields of Physics, Chemistry, Biology, Material Science, Health Science and Engineering. Nano Composite made up of Fibrin, Graphene Oxide and Iron Oxide. Nano Particals are synthesized by a variety of methods depending upon the particles and its final application. The methods can be classified as physical, chemical or biological. Physical techniques (mainly top-down techniques) include attribution or mechanical milling, thermal and vapour gas decomposition (Castro and Mitchell, 2009) and laser ablation synthesis in solution (LASIS) (Amendola and Meneghetti, 2009). Chemical synthesis involves bottom-up techniques. In Micro-emulsion/micelle based techniques the introduction of microwave and ultrasound irradiation into a reaction system has been employed. Bovine blood was hygienically collected from a municipal slaughter house at Chennai, India. It was mechanically stirred with glass rod to isolate the fibrin, which was in crude form. Crude fibrin was washed thoroughly with cold distilled water and treated with 0.5 M Sodium Acetate and 30% Hydrogen Peroxide. About 5 mg of iron oxide was dissolved in 10 ml distilled water and sonicated for 30 minutes. 5 mg of graphene oxide was dissolved in 10 ml distilled water and sonicated for 30 minutes. Both the sonicated samples were mixed together and kept for stirring for 2 h. Then 5 ml of fibrin solution was added to the above sonicated solution.. A black color residue with precipitate particle was obtained.