Prediction of Antigenic Peptide and MHC Binder from ITX-3 Tegenaria agrestis: Current Approach for Synthetic Vaccine Development

Sherkhane AS and Gomase VS

Abstract

Venom of Tegenaria agrestis species causes necrosis in humans. ITX-3 is a toxin with 68 amino acids. Antigenic peptides of Tegenaria agrestis toxic protein are most suitable for synthetic peptide vaccine development because with single epitope, the immune response can be generated in large population. In this research, we used PSSM and SVM algorithms for the prediction of MHC class I & II binding peptide, antigenicity, Solvent accessibility, polar and nonpolar residue to analyze the regions that are likely exposed on the surface of proteins which are potentially antigenic that allows potential drug targets to identify active sites as well as to design synthetic peptide vaccine.