Original Articles
Andi Emelda
Abstract
Antioxidants are compounds that can prevent or inhibit the oxidation process. This study was conducted to determine the total polyphenol, test the antioxidant activity against free radicals (DPPH), determine IC50 of ethanol extract from several cocoa(Theobroma cacao) clones that is KDI-3, PA-300, PA-310, and NA-34. The study samples were made with unfermented condition. Each clone was extracted with hexane and ethanol 70%. The results showed polyphenol total of ethanol extract from cocoa seed for KD1-3, PA-300, PA-310, and NA-34 are 84 mg/g, 65.92 mg/g, 86,11mg/g, and 49,25mg/g respectively. The IC50 for KDI-3, PA-300, PA 310, and NA-34 are 9.30 pm, 8.50 ppm, 6.40 ppm, and 10.23 ppm respectively. The results described each clone of cocoa beans has a different total polyphenol content, antioxidant activity against free radicals, and IC50, but they still showed the percentage inhibition of DPPH free radicals above 80 % at a concentration of 25 ppm and showed a very strong antioxidant activity with IC50<50. The total antioxidant activity and the highest polyphenol were indicated by the PA-310 clone.