Reena Tondwal and Man Singh
Abstract
A comparative study of chitosan derivative with methionine (CM) and trimesoyl 1, 3, 5-tridipropyl malonate ester (TTDPM) as drug delivery vehicle encapsulated with the model drug ( Methotrexate: MTX) individually, for breast cancer drug delivery system. MTX-TTDPM and MTX-CM were prepared using a 1 : 1 ratio of MTX, TTDPM and MTX, CM in acetone and ionotropic gelation medium at RT followed by 24 and 3 h respectively. MTX interact with TTDPM and CM by hydrogen bonding confirmed through FTIR and further characterized by SEM, HR-TEM and DLS for their morphological and narrow particle size distribution respectively. TTDPM and CM has functionality which assistances to bind MTX anticancer drugs for their imminent use in breast cancer drug delivery system. Effects of TTDPM, CM and their complexes with MTX were studied for MTX release in phosphate buffer saline (pH 7.4) + 10% DMSO and phosphate buffer saline (pH 7.4) respectively. H3C CH3 H2C H2C CH2 CH2 O O C C O CH O O C H C CH3 OH H2C O OH H2C O O OH H2C O O HO 3 CH2 O H2C O H2C O H2C HO HO RT, 24 h O O OH NH2 N H N O N N N N NH2 NH NH C C C C O O C n O C CH O O CH C O O CH2 H2C H3C O C O CH2 CH2 CH3 HC NH2 H2C CH2 S CH3 OH CH3 Dendrimer (TTDPM) H3C H2N MTX Chitosan Derivative (CM) H2C CH3 Interaction Electrostatic Interaction CH N NH2 H2C O 2 CH2 O O OH OH OH OH OH H2N N N N N OO HN OH C C O HC N O O O H C HHN N N H2C HO H2C O O HO H2C O O O HO OH O N N N NH2 NH NH O H C H C H2N CH2 CH3 O C O C OH HC CH 3 2 H C O O CH2 OH NH2 3 2 O C C HC O O C HC O HC O OH OH O O O NH H2C CH2 OH OH O O C O HO OH O OH HN O CH O O NH O CH O H2N N N N N O N H2N N N N N H N S CH3 H2N N O NH N N 2 CH2 N H N 2 N N H2C H3C N N N N N NH2 H2N 2 H2C CH3 H2N MTX-Dendrimer Complex MTX-Chitosan Derivative