Phytochemicals in Brassica juncea L. seedlings under imidacloprid-epibrassinolide treatment using GC-MS

Anket Sharma, Vinod Kumar, Suk

Abstract

Brassica juncea L. seedlings were screened for the presence of compounds of medicinal importance under imidacloprid (IMI) stress in binary combination wit h 24-epibrassinolide (24-EBL). The compounds were i dentified using gas chromatography-mass spectroscopy (GC-MS). The compounds identified were 4,5-epithiovaleronit rile, 2- naphthoic acid-methyl ester, L-(+)-ascorbic acid 2, 6-dihexadecanoate, phytol, 9,12-octadecadienoic aci d (Z,Z), 9- octadecenoic acid, alpha-tocopherol, ergosta-5,22-d ien-3-beta-ol, campesterol and gamma-sitosterol. Mu ltiple linear regression (MLR) analysis of data revealed t hat increase in IMI concentrations results into dec line in the concentrations of these compounds, whereas seed pre -soaking with 24-EBL significantly decreased the IM I toxicity and enhanced the phytochemical concentrations.

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