Phytochemical study of ethyl acetate extract and antioxidant activity of Genistaquadriflora Munby (Fabaceae)

Research Article

R. Boukaabache, N. Boubekri, O

Abstract

This study concerned a chemical screening of the aerial parts of GenistaquadrifloraMunby(endemic species in Algeria and Morocco), in which the presence of several chemical groups was revealed (flavonoids, isoflavonoids, triterpens and others). The chromatographic study on silica gel of the ethyl acetate soluble part of the aqueous- MeOH extract of the aerial partsof this plant, allowed the isolation and the structural elucidation offive known compounds: thevetiaflavone (1), genkwanin (2), genistein (3),biochanin A (4) andisoprunetin (5). The structures were established on the basis of physical and spectroscopic analysis, and by comparison with the literature data. Antioxidant properties were assayed in terms of antioxidant activity of chloroform and ethyl acetate soluble parts. The scavenging ability on 1, 1-diphenyl-2-picrylhydrazyl (DPPH), is determined with spectrophotometric method.

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