Original Articles
Dinesh Kumar, Anupama Singh, E
Abstract
Plants are known to possess compounds having therapeutic, anti-inflammatory as well as anti-bacterial properties. So the present work was planned to analyze phytochemical constituents and screening of antimicrobial properties of methanolic extract of Psidium guajava and Nyctanthes arbor-tristis against two isolated mouth pathogenic bacterial strains. A total of 30 oral swabbed samples were collected from Institute of Biosciences and Biotechnology, Chhatrapati Shahu Ji Maharaj University campus, Kanpur (U.P.), India from participants. After culturing, two strains were characterized and found to be Enterococcus faecalis and Staphylococcus aureus. Characterization of isolates was done by morphological and biochemical tests. The above isolated strains were tested for their sensitivity towards the Psidium guajava and Nyctanthes arbor tristis methanolic leaves extract by disc diffusion method. The presence of Phytochmicals determined by qualitative phytochemical tests. The highest antimicrobial properties were shown by methanolic extract of Psidium guajava with Staphylococcus aureus at concentration 100%, 50%, 25% and 12.5% with zone of inhibition having diameter of 10.5 ± 0.50 mm, 9.7 ± 0.25 mm, 8.7 ± 0.68 mm and 7.6 ± 0.36 mm respectively while with Enterococcus faecalis, zone of inhibition having diameter of 9.4 ± 051 mm, 7.9 ± 0.40 mm, 7.3 ± 0.25 mm and 5.8 ± 0.25 mm respectively in comparison to methanolic extract of Nyctanthes arbor-tristis