Original Articles
M Rammika
Abstract
Folin-ciocalteu method was optimised and tested on panicum maximum grass samples and results showed that the method is reliable and consistent. Optimization was done in terms of volume of sample aliquot, volume of folins reagent, sodium carbonate, wavelength, extractant and ratio of volume of water to acetone in extractant. The volume of sample aliquot was found to be 0.1, while the volume of folins reagent was found to be 0.5 ml and the volume of sodium carbonate was found to be 2.5. 760 nm was found to be the best wavelength with 60% acetone being better to 50% methanol as an extractant. The quantity of tannins in panicum maximum grass was found to range from 0.63-0.67 μg/mL with an average of 0.65 μg/mL and the limit of detection was found to be 0.32 μg/mL while the limit of quantification was found to be 0.96 μg/mL. Statistical Analysis System (SAS) was used to analyze data and the coefficient of variation was 12.6 with a standard error of 0.009369 and a standard deviation of 0.09554.