Optimization of Solid State Fermentation Conditions for the Production of Cellulase by Using Trichoderma Reesei

Moses Jeyakumar Rajesha, Leela

Abstract

The enzyme cellulase is produced by utilizing contemptible substrates like rice bran and corn straw supplanted with nutrient sources in Solid State Fermentation using the fungal strain Trichoderma reesei (MTCC *164). A fermentation time of 6 days is required to obtain maximum Cellulase and β-glycosidase activity. The activity decreases with further increase in fermentation period. The optimum rice bran- corn straw ratio, pH and temperature for maximum production of Cellulase are found to be 5:5, 5 and 28°C respectively. The crude enzyme is partially purified by Ammonium sulphate Precipitation, Dialysis and finally Ion Exchange Chromatography. The effect of temperature, pH and substrate concentration were also studied in the purified sample and the optimum condition for enzyme activity is found to be pH 5.0, temperature 50° C.