Optimization of Conditions and Production of Carboxy Methyl Cellulase by Bacteria Isolated from Higher Termite Soil

Sreeremya S*, Nishaa S and

Abstract

The current work deals with the studies of isolation and preliminary characterization of the bacteria isolated from higher termite soil. Termites play an important role in the turnover and mineralization of complex biopolymers, such as wood and other cellulose and hemicelluloses containing materials. Lignocellulose is the most predominant component of the woody and dead plant materials, as well as it is the most abundant biomass on earth, especially in terrestrial ecosystems. Thus the work focuses on using lingo cellulosic waste efficiently for production of Carboxy Methyl Cellulase (CMC). As CMCase have higher hydrolyzing capacity than the other two cellulases, cellobiase and cellobiohydralases it has wide application in industry such as bread and baking industry, laundry etc. Thus the work focuses on identification of efficient bacterial species in this work 6 bacterial species were isolated among which I4 produced higher CMCase enzyme. After the morphological, biochemical and identification test, the I4 isolate was found to be Bacillus sp.

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