Original Articles
R. D. Kaushik, Payal Rathi, Ma
Abstract
The MnII catalysed oxidation of Doxycycline Hyclate(DCH) by periodate ion in aqueous medium was followed by monitoring the increase in the absorbance of reaction intermediate and made the basis for developing newer method for microgram estimation of DCH. The reaction is first order with respect to substrate and oxidant each. The best fit conditions developed for a new and simple kinetic-spectrophotometric method for microgram determination of DCH in the range 51.30 μg/mL – 615.54 μg/mL are, [NaIO4] = 1.0 × 10-5 mol dm-3, [MnII] = 1.0 × 10-3 mol dm-3, Temperature = 35.0 ± 0.1°C, pH = 5.0 and λmax = 486 nm. The characteristics of various calibration curves, percentage recovery, effect of excipients, correlation coefficient and comparison with other reported methods are presented.