Multidrug Resistant-Proteus Mirabilis Isolated from Chicken Droppings in Commercial Poultry Farms: Bio-security Concern and Emerging Public Health Threat in Bangladesh

Arifatun Nahar, Mashuk Sidd

Abstract

Poultry is a rampantly expanding agro-industry in Bangladesh like other developing countries. Several studies have detected multidrug resistance (MDR) Proteus mirabilis from poultry meat globally; however, no similar data was available for poultry samples in Bangladesh. P. mirabilis is a zoonotic human pathogen of urinary tract infection (UTI), nosocomial infection and wound infection, therefore, a potential threat to public health. We isolated P. mirabilis from chicken droppings collected from local commercial poultry farms and examined their antimicrobials susceptibilities. Chicken droppings were streak-cultured onto xylose lysine deoxycholate agar plates after enriching in buffered peptone water. Selective colonies were identified by biochemical test and API20E kits. Antimicrobial susceptibilities were tested by Kirby-Bauer disk diffusion method. Total 36 P. mirabilis were isolated from 39% (27 of 70) chicken droppings. Tetracycline evidenced as the highest individually-resistant (94%, 34/36) antibiotic (AB) while ciprofloxacin was the lowest (17%, 6/36). Hazard lies when 83% P. mirabilis were proved to be MDR (30/36), being resistant to three or more AB. Findings provide a baseline data on MDR P. mirabilis circulating around these PFs, it would assist the veterinarian in rational treatment and biosafety planning. More detail studies will be required to clarify their antimicrobial resistance and clinical relevance.