Molecular Diagnosis of Helicobacter Pylori Strain by 16S rDNA PCR Amplification and Direct Sequencing

Hirendra nath Banerjee, Mon

Abstract

Aim: Rapid detection of H. pylori strains by PCR-Sequencing. Methods: 16S rDNA amplification by PCR from template genomic DNA,confirmation of amplicon size by agarose gel electrophoresis ,sequencing of amplicons by automated sequencer,analysis of sequences by NCBI -BLAST software. Results: The PCR -Sequencing and analysis of the sequence data by BLAST resulted in detection of the strain to be of H. pylori strain#26695. Conclusion: The pathogenicity of H. pylori depends on the strain of the bacteria, PCR-Sequencing and analysis of the sequence data by BLAST can be a very quick and useful diagnostic method of the pathogen.