Molecular Characterization of a Squalene Synthase Gene from Phellinus igniarius

Lin Weiping

Abstract

According to the conserved sequence of SQS（squalene synthase） from NCBI database, designed primers and amplified SQS gene fragment with RT-PCR product from Phellinus igniarius total RNA. Then obtained full length SQS gene using the RACE technology. Analyzed the amino acid sequences, and constructed phylogenetic tree. The SQS cDNA was constructed into the prokaryotic expression vector pET22b and expressed in E.coli BL21(DE3). SDS-PAGE Analyzed protein expression. Lay the foundation for further analysis of the structure and function of squalene synthase.