Original Articles
Yu Duan
Abstract
The research on the miscibility of the mixed monolayer of unsaturated phosphatide and flavonoids can reveal the mechanism of molecular interactions, which is important for bioactive mechanism of action of flavonoids. An amphiphilic rutin stearate (RS) was obtained by esterification of rutin with stearic acid catalyzed by immobilized Candida antarctica lipase B (Novozym 435), and the result suggested that the regioselectivity of the lipase-catalyzed esterification of rutin was specific at the C4’’’ position of the rhamnose moiety. The mixed monolayer of L-α-di-(cis-9-octadecenoic acid) phosphatidylcholine (DOPC) and RS was spread at the air/water interface and its miscibility at subphase temperatures of 37℃ and 10℃ were investigated by Langmuir film balance. The two components of DOPC/RS mixed monolayer were miscible throughout the mixture composition range, and the better miscibility was obtained at higher subphase temperature, i.e., 37℃. At both subphase temperatures of 37℃ and 10 ℃, the condensing effect of RS was most prominent at the lower surface pressure. The condensing effect could be ascribed to the match of RS and DOPC molecular shapes during the monolayer formation, and the molecules of two components might occupy smaller areas.