Rupesh Dudhe, Pramod Kumar Sha
Abstract
A rapid and sensitive U.V. visible spectroscopic method was developed for the estimation of pyrimidine 3,4-dihydro-1-(tetrahydro-3,4-dihydroxy-5- (hydroxymethyl) furan-2-yl) -6-(4-nitrophenyl)-4-phenylpyrimidin-2(1H)-one (6N) in bulk form. Pyrimidine derivative was monitored at 314 nm with U.V. detection and there is no interference of diluents at 314 nm. The method was found to be linear in the range of 10 -50 mcg/ml. The accuracy and precision were determined and validated statistically. The method was validated as per International conference on harmonization [ICH] guideline. The results showed that proposed method is suitable for the accurate, precise and rapid determination of pyrimidine derivative.