Original Articles
Jenny Sushmitha Evangiline D.*
Abstract
The present research work discusses the development of visible spectroscopic method for the estimation of Tramadol Hydrochloride. The optimum conditions for the analysis of the drug were established. The maximum wavelength (λmax) was found to be 749nm. The validation was performed as per ICH guidelines for linearity, accuracy, precision, LOD and LOQ. The method shows high sensitivity with linearity in the range of 10-50μg/ml and shows a linear relationship between the absorbance and concentration with coefficient of correlation 0.998. The regression of curve was Y = 0.014x+0.016. The precision of method was found to be good. The percentage recovery was found to be 99.15±0.0950.The optimized method showed good reproducibility and recovery with RSD <2%. The proposed method will be suitable for analysis of Tramadol Hydrochloride in bulk as well as pharmaceutical formulations in quality control purpose. It is thus concluded that the proposed method is new, simple, cost effective, safe, accurate, precise and environmental friendly.