Research Article
Himanshu Tripathi, Rojison Kos
Abstract
Chlorogenic acid is commonly employed as a biomarker of Coffea arabica and responsible for some of important pharmacological activity. The quality control of chlorogenic acid in herbal product was important by using High Performance Liquid Chromatography (HPLC). An attempt was made to develop and validate an analytical method by using isocratic elution program with less solvent consumption and less run time for Coffea arabica extract using HPLC. The present study described a developed method used a Purospher reverse phase C18 end capped column (250× 4.6 millimeter; 5 micrometer) using an isocratic elution of potassium dihydrogen orthophosphate with acetonitrile as mobile phase with a flow rate of 1.0 ml/min and chlorogenic acid was monitored at 274 nm. Method has been successfully validated for linearity, specificity, precision, accuracy, range and ruggedness with good recoveries. Specificity of the method was assessed using PDA (Photo diode array) profile and peak purity was greater than 99%. Calibration curve exhibited good linear regression (r2> 0.999) with in the test range and recovery was 96- 102%.