Liquid-liquid extraction method for Ziprasidone (ZRS) bioanalysis by using ZRS-D8 (stable isotope) as internal standard

Rajesh Dhiman*, Brijesh Kumar,

Abstract

A selective, sensitive, liquid chromatography mass spectrometry/ mass spectrometry (LC-MS/MS) method was developed and validated for the quantification of Ziprasidone in human plasma by using Ziprasidone D8 as internal standard. Sample preparation process was accomplished by liquid-liquid extraction technique. The processed sample was chromatographed and analyzed on Zorbax Eclipse XDB-C18,100×4.6 mm (3.5μm) column using mobile phase [Acetonitrile: Buffer-1 (85:15%, v/v)] and 50% Acetonitrile in water as diluent. Ziprasidone was chromatographed and analyzed by MS Detector. This method shows good linearity over a range of 0.2980 ng/ml to 201.4820 ng/ml. Signals from the detector were captured in a computer and processed using Mass Lynx version 4.1SCN 843 software.