Research Article
Kumar J* and Melo JS
Abstract
Immobilization of enzymes on a variety of different matrices has always attracted researchers to improve the functionality, stability and reusability and its application in bioprocess and biosensors. Present study illustrates a new biological material, jackfruit membrane, as a natural support for immobilization of the urease enzyme for optical detection of urea. A simple protocol was optimized for immobilization of urease enzyme on the membrane. Km of immobilized urease (13.8 mM) was less than that of free urease (39.24 mM). SEM and FTIR studies of the urease immobilized membranes were carried out to characterize the changes after immobilization. Immobilized urease has shown good reusability and stability. Further, immobilized urease was applied for detection study and a detection range between 0.5-10 mM urea was estimated from the linear range of the calibration plot.