Research Article
Saganuwan AS, Patrick A , N
Abstract
Combination of column chromatography, thin layer chromatography and neutron magnetic resonance spectroscopy can be used for isolation and characterization of phytochemical principles. The study was carried out to further re-assess the credibility and the problems associated with the methods using methanolic extract of Abrus precatorius leaf. Column and thin layer chromatography led to separation of two n-hexane isolates, H1 (yellow with Rf=0.121) suspected to be alkaloid. But the spectra may be flavonoid, quinone, flavanquinone and glycoside and H2 (bluish-green with Rf=0.608) suspected to be mixtures of aliphatic and aromatic compounds in addition to methanold4. Neither of the isolates contained a single compound in sufficient purity for further assessment.