Isolation of plant growth promoting substance producing bacteria from Niligiri hills with special reference to Phosphatase enzyme

D. Saravanan,M. Radhakrishnan

Abstract

The present study isolated plant growth promoting substance producing bacteria from Niligiri hills, Ooty, Tamilnadu. Totally 17 morpholigaclly different bacterial isolates were isolated. All the isolates were screened for plant growth promoting substances. Among 17 isolates, 5 (29.4%), 9(33.3%) and 11(40.7%) shows positive results for ammonia, acetoin and phosphate solubilization, 9 isolates (33.3%) produced indole acetic acid and 7 (25.9%) isolates shows positive for N2 fixation. The strain NG7 selected as potent strain for the production of phosphatase since the strain showed best activity among other strains. The NG7 strain produce 27.6U/ml of phosphatase enzyme in production. The strain NG7 showed good production of phosphatase when glucose (carbon source), beef extract (nitrogen source), pH 7, temperature 25ºC at 72hrs of incubation of production medium. The potent strain NG7 was identified as Bacillus sp.