Research Article
Syukria Ikhsan Zam, Syamsuardi
Abstract
The endophytic bacteria has variety of roles, includes roles in plant protection. This study aimed to isolate and characterize endophytic bacteria from Citrus aurantifolia Swingle leaves, and tested the antifungal activity towards Fusarium oxysporum. Isolation of endophytic bacteria was done using spread plate method. Test of antifungal activity was carried out by growing each of endophytic bacteria in Tryptic Soy Broth medium for 24 hours cultivation at temperature of 27 oC and agitation of 120 rpm, further test was using agar diffusion method. Characterization of endophytic bacteria was done through observation of colony morphology, Gram staining and analysis of 16S rRNA. Results showed that 4 endophytic bacteria has been isolated. The 16S rRNA analysis indicated bacteria isolates obtained consecutively were Bacillus cereus RNS-01, Pantoea agglomerans ZFJ-15, Bacilus subtilis 55C1-1 dan Bacillus pumilus SH-B11. Isolates CA1 and CA2 does not has antifungal activity towards Fusarium oxysporum, while CA3 and CA4 showed antifungal activity.