Isolation and structure elutidation of coumarin derivate from fruit seeds of Clausena excavata

Adlis Santoni*, Sanusi Ibrahim

Abstract

This study was conducted to obtain the structure of coumarin compound by extracting the seeds from the fruit powder Clausena excavata with dichloromethane and then separated by column chromatography. Fractions obtained from the separation process then purified by preparative TLC and isolated compound were obtained as 25.67 mgrams. Elutidation structure of isolated compound performed using UV spectrophotometer, IR, 1H-NMR, 13C-NMR, 2D-NMR (HMBC and HMQC), GC-MS, and also through a literature search was proposed, the isolated coumarin derivatives with the name: 7-hydroxy-6-methyl-coumarin.