Isolation and Screening Protease Acid, Neutral and Alkaline Producing Bacteria from Dadih (Traditional Indonesian Food)

Anthoni Agustien, Zita Nabila

Abstract

Protease is an enzyme that is economical and very widely used in various fields of industry. This study aims to isolate and screen the bacteria producing acid, neutral and alkaline protease enzymes from the dadih. Isolation of bacteria from the dadih using the method plate count while screening bacteria producing protease enzymes by using Skim Milk Agar selection medium at various levels of acidity. The results showed that 111 bacterial isolates from dadih, 27 isolates of bacteria produce protease, where 5 bacterial isolates have the potential to produce acid protease, 6 isolate of protease neutral and 16 isolates as alkaline protease producer.