Isolation and Characterization of Tyrosinase (A Carbon Trapping Enzyme) Producing Microorganisms, in the Agricultural Soil of Western Uttar Pradesh and the Study of Enzymatic Activity of Tyrosinase Produced

Prashant Agarwal, Silky Sharma

Abstract

Tyrosinase is an enzyme which shows a carbon trapping activity in soil. Carbon, being one of the main component of the greenhouse gases needs to be reduced in the earth’s atmosphere. Hence, tyrosinase producing microorganismin the soil plays a vital role in reducing carbon content and hence the global warming. Taking in view the importance of tyrosinase producing microorganism in the soil, present study is conducted to isolate and characterize the microflora which is the potent producer of tyrosinase in the soil of western Uttar Pradesh. The work dealt with the growth of soil dwelling microorganism on suitable growth medium. Once they were characterized as enzyme producer, the enzyme was produced and crude enzyme was then extracted. Finally, the activity and kinetic studies of the enzyme were performed from a very potent bacterial population of micromonospora found among them.