Research Article
K.Krishna Chaitanya, D.Gowri S
Abstract
A simple isocratic RP-HPLC method was developed and subsequently validated for the analysis of Isradipine in pharmaceutical dosage forms. Separation was achieved by using kromasil C18 (100 x 4.6mm, 5μm) column with flow rate of 1.7ml/minute and Analytes were monitored by UV detection at 326 nm, using a mixture of mobile phase containing 500 ml of water, 400ml of methanol and 100ml of tetra hydro furan mix well and sonicate to degas it. The retention time for Isradipine was found to be 8.95 minutes. Calibrate curves for Isradipine was linear over the concentration range 50-400 μg/ml with correlation coefficient 0.999. The percentage estimations of the Isradipine in market formulations by RP-HPLC were found in between 99.61-99.86%. The developed method was validated according to ICH guidelines and values of accuracy, precision and other statistical analysis were found to be in good accordance with the prescribed values. The recovery of the drug by standard addition method was found in range of 99.99-101% LOD and LOQ were 0.003μg/ml and 0.01μg/ml respectively. Thus the proposed method was found to be accurate, precise, reproducible and specific and can be successfully applied for simultaneous quantification of Isradipine in pharmaceutical dosage forms for future.