Innate immunological activity of immunoglobulin Y towards Parthenium hysterophorus leaf proteins

Praveen Kumar Vemuri, Bhavana

Abstract

Considering the augmented importance of immunoglobulin’s role in allergic reactions and the underlying mechanisms of IgY in allergic conditions, the objective of our study is to purify proteins from Parthenium hysterophorus leaves, IgY antibodies from chicken eggs and to check the immunological activity of the antibodies with Parthenium hysterophorus allergens. Phytochemical analysis of the constituents in leaves of Parthenium hysterophorus plant revealed the presence of glycosides and proteins. To elucidate which proteins have been extracted and thus could contribute to immunological activity, they were subjected to SDS-PAGE analysis by various staining methods. Later, the leaf proteins were screened for their activity against antibodies for allergic responses by using ELISA technique, which proved that they are capable of triggering immunological responses by IgY.