Original Articles
S. Usharani and R. Anuradha
Abstract
Many oxidative stress related diseases like cancer, diabetes, neurodegenerative disorders and cardiovascular diseases are occurring as a result of accumulation of free radicals in the body. Lots of researches are going on worldwide towards finding natural antioxidants from plant source. In the present study the antioxidant activity of methanolic extract of Wattakaka volublis leaf was investigated using various in vitro assays. 1,1-diphyenyl -2-picryl –hydrazyl,(DPPH), superoxide, nitric oxide and hydroxyl radical scavenging activity of the extract was determined at different concentrations and compared with standard antioxidants, such as BHT(butylated hydroxyl toluene), quercitin, 1- ascorbic acid. DDPH radical, superoxide, hydroxyl radical and nitric oxide radical scavenging effects of the extracts at different concentrations were evaluated following standard methods. The radical scavenging activity was concentration dependent, DDPH showing maximum percent inhibition(83.44%) at 200mg/ml . Similarly, hydroxyl, superoxide and nitric oxide scavenging potential also at their maximum at higher concentrations. superoxide radical inhibition was 80.04% at 200 mg/ml and 70.54% inhibition of nitric oxide was noted at 200 mg/ml. Hydroxyl scavenging effect was 86.96% at 200 mg/ml. The results indicated that methanolic extract of Wattakaka volubilis possess good natural antioxidant may be used to control some dangerous diseases.