In vitro antimicrobial and antioxidant activities of Pimpenella tirupatensis leaf methanolic and ethyl acetate extracts

P. Ranjit* and Archana Giri

Abstract

In-vitro antimicrobial and antioxidant activities of Pimpenella Tirupatensis Methanol and Ethyl acetate leaf extracts concentrations ranging from 50 to 150 μg/mL were analyzed on different clinical bacterial strains (Klebsiella pneumoniae, Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and Salmonella typhimurium, Proteus mirabilis, Bacillus subtilis, and Proteus vulgaris), using agar disc diffusion method and broth dilution method (MIC and MBC determination) for antimicrobial activity and DPPH (1,1-diphenyl-2- picrylhydrazyl) assay, Nitric oxide scavenging assay, Ferrous reducing antioxidant power (FRAP) for antioxidant activity. The extracts showed maximum inhibitory effect against K. pneumonia, Proteus mirabilis and Pseudomonas aeruginosa, with no activity against S. typhimurium and Proteus vulgaris among the bacterial strains. The antioxidant activity showed that the extracts exhibited scavenging effect in concentration-dependent manner on superoxide anion radicals and hydroxyl radicals leading to the conclusion that the plant has got a broad spectrum antimicrobial and antioxidant activity and could be a potential alternative for treating various diseases.