Identification of Binding Affinity of TAP Peptides for Development of Fragment based Vaccine from Long neurotoxin 2 [N. naja]

Sherkhane AS and Gomase VS

Abstract

Long neurotoxin 2 protein from the venom of Naja naja shows a great affinity towards acetylcholine receptors, which produces peripheral paralysis by blocking neuromuscular transmission at the postsynaptic site. In this study, we use a cascade SVM based TAPPred method which found 26 High affinity TAP Transporter peptide regions. TAP is heterodimeric transmembrane protein which transports antigenic peptide proteins to the endoplasmic reticulum where it binds to MHC class I molecules, These MHC-peptide complexes will be translocated on the surface of antigen presenting cells (APCs), where they are recognized by T-cell receptors to elicit an immune response. Therefore, prediction of TAP-binding peptides is helpful in identifying the MHC class I-restricted T-cell epitopes and hence helpful in the designing of synthetic peptide vaccine.